Polymer length of teichuronic acid released from cell walls of Micrococcus luteus
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منابع مشابه
Polymer length of teichuronic acid released from cell walls of Micrococcus luteus.
Teichuronic acid released from its phosphodiester linkage to peptidoglycan in the cell walls of Micrococcus luteus by mild acid treatment is resolved into a ladderlike series of bands by electrophoresis on polyacrylamide gels in the presence of borate. Each band of the ladder differs from its nearest neighbor by one disaccharide repeat unit, ----4)-2-acetamido-2-deoxy-beta-D-mannopyranuronosyl-...
متن کاملReactions of second stage of biosynthesis of teichuronic acid of Micrococcus lysodeikticus cell walls.
A polysaccharide of alternating glucose and N-acetylmannosaminuronic acid residues is formed in the second stage of teichuronic acid biosynthesis by the particulate enzyme fraction of Micrococcus lysodeikticus. Di(N-acetylmannosaminuronylj-N-acetylglucosaminyl carrier lipid, the third intermediate formed in the initial stage of teichuronic acid synthesis, serves as an acceptor for the sequentia...
متن کاملBiosynthetic elongation of isolated teichuronic acid polymers via glucosyl- and N-acetylmannosaminuronosyltransferases from solubilized cytoplasmic membrane fragments of Micrococcus luteus.
Cytoplasmic membrane fragments of Micrococcus luteus catalyze in vitro biosynthesis of teichuronic acid from uridine diphosphate D-glucose (UDP-glucose), uridine diphosphate N-acetyl-D-mannosaminuronic acid (UDP-ManNAcA), and uridine diphosphate N-acetyl-D-glucosamine. Membrane fragments solubilized with Thesit (dodecyl alcohol polyoxyethylene ether) can utilize UDP-glucose and UDP-ManNAcA to e...
متن کاملThe Cell Wall Teichuronic Acid Synthetase (TUAS) Is an Enzyme Complex Located in the Cytoplasmic Membrane of Micrococcus luteus
The cell wall teichuronic acid (TUA) of Micrococcus luteus is a long-chain polysaccharide composed of disaccharide repeating units [-4-β-D-ManNAcAp-(1→6)α-D-Glcp-1-](n), which is covalently anchored to the peptidoglycan on the inner cell wall and extended to the outer surface of the cell envelope. An enzyme complex responsible for the TUA chain biosynthesis was purified and characterized. The 4...
متن کاملA polymer containing glucose and aminohexuronic acid isolated from the cell walls of micrococcus lysodeikticus.
The surveys of Cummins & Harris (1956, 1958) established that the cell walls of Gram-positive bacteria all contain the amino sugars glucosamine and muramic acid (3-O-a.-carboxyethylglucosamine) and the amino acids alanine, glutamic acid and either lysine or aci-diaminopimelic acid. In some species either glycine or aspartic acid was also present, whereas the other amino acids found in proteins ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1990
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.172.9.5154-5159.1990